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This consists of a combination of solvents that are used to disperse the samples throughout the entire system. All of the solvents must be miscible in the solution. Otherwise, the immiscible solvents will trigger pressure build-up within the High Performance Liquid Chromatography system. The analysis length and the separation of compounds are affected by the ratios of every solvent component within the mobile phase.

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The stationary phase is often referred to as the 'column'. This portion of the HPLC system is considered to be the 'heart of separation'. It derives from securely packed material within a stainless steel column. High pressure is needed in order to disperse the solvents throughout the system. This is often referred to as 'high pressure liquid'.

The detector is a unit that recognizes the departed compounds within the sample. There are an assortment of detectors that utilizes various modes of detection, such as refractive index, fluorescence, mass spectroscopy and ultra-violet rays.

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Doing a warm up and cool down effectively doesn't add that much time onto your workout, but it can ensure that you do not injure yourself or have to limp through the rest of your day or night with painful cramps.

Using the same light activities as your warm up, take 10 minutes after your workout to let your heart rate, blood pressure and body temperature drop before sitting in your car to head home, hopping in the shower, etc. If you have been sleeping or sitting down for a length of time, your muscles are tight and cold. Your body is at rest and needs to gently be awoken and eased into the workout that is about to come. After you have completed your workout, spend at least a few minutes cooling down to allow the more aggressive blood flow to scale back to normal. Your heart has to work much harder to keep the body fully supplied with nutrients and blood during your workout, but when you stop there is a reduced need for blood flow.

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When samples are dispersed through the column, all of the compounds within the sample will interact at the same time. It interacts with the mobile and stationary phase in order to output a different elution time for every compound. The purpose of each analysis is to divide the peak of interest from all the other remaining compounds.

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