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Heat map of Rec-seq enrichment values for wild-type Cre showing the log2 of the enrichment worth for each nucleotide at every place in loxP relative to the canonical base (black define). The outcomes of Rec-seq experiments on wild-type Cre (black dots) and variants thereof (colored dots) visualized using t-SNE multi-dimensional proximity analysis37, exhibiting that experimental replicates are clustered by similarity across all specificity options. On the left, you possibly can see the outcomes of the house crew, and on the fitting, the outcomes of the away staff. To visually signify one such map, we used the t-SNE algorithm37 to correlate the results of particular person Rec-seq experiments utilizing multi-dimensional similarity evaluation (Fig. 2g). The proximity of experiments within the t-SNE visualization relates their similarity throughout the total Rec-seq profile. We discovered no important differences of enrichment values when performing Rec-seq experiments with a more highly mutagenized loxP library (Supplementary Fig. 1). Additionally, the core sequence of loxP was held constant because the core areas of two recombining loxP substrates must be complementary29

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