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The Four Biggest 2018 New Betting Sites Mistakes You can Easily Avoid

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작성자 Ivory
댓글 0건 조회 16회 작성일 25-02-10 16:48

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Double Ala substitution at each Gln90 and Gln94 performed equally to the Gln90→Ala single mutant (Fig. 2e), suggesting that the DNA-contacting residue Gln90 plays the dominant position in defining DNA specificity amongst the 2 residues. In addition, Rec-seq identified a contribution from a secondary residue previously unknown to take part in specifying positions 5-7. Ala substitution at Gln94 resulted in decrease specificity at positions 6 and 7 however compensatory increases elsewhere (Fig. 2e), regardless that Gln94 does not directly contact the DNA, however as a substitute engages in hydrogen bonds with Gln9035. Rec-seq revealed the sequence preference of Cre to be asymmetric, as is obvious when the left and right half-site enrichment profiles are superimposed (Supplementary Fig. 6a). To ensure that an asymmetric sequence desire is a property of the enzyme and not due to the totally different DNA sequences flanking the library oligonucleotides (Fig. 1b), we performed Rec-seq using a substrate library equivalent to the unique besides that the non-palindromic loxP core was changed with its reverse complement (Supplementary Fig. 6b). The Rec-seq enrichment profile of this "inverted core" loxP library mirrored, fairly than duplicated, the profile on the original substrate library (Supplementary Fig. 6b), indicating that the oligonucleotide sequence context was not liable for the asymmetry of the Cre specificity profile

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